Coating plastics, binding capacity

Two-Site Assay Protocol for the Measurement of Human a-Fetopro-tein. Purified antibodies to human a-fetoprotein (AFP) are prepared by allowing 1 ml of high titer antiserum (binding capacity approximately 125 p,g of AFP per milliliter) to react with 1 mg of AFP immunoadsorbent (protein uptake 120 p,g per milligram of cellulose) for 48 hr at 4°. Elution of high affinity antibody is carried out as described above for the preparation of purified insulin antibody. The antibody solution is diluted to 100 ml in 50 vaM sodium hydrogen carbonate buffer (pH 9.6) and used to coat 500 plastic tubes as described above. 

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