Clostridium pasteurianum. hydrogenase

Figure 8.1 The active center of Clostridium pasteurianum hydrogenase.

Fig. 4. H2 photoevolution from ascorbate via P. laminosum PSI particles under different conditions of immobilization. The complete assay system contained 100 mM Mes-NaOH buffer, pH 7.0 75 mM sodium ascorbate 15 mM DTT 2 mM TMPD 1% (w/v) BSA PSI particles (30 pg Chi) 50 pi (saturating amount) of Clostridium pasteurianum hydrogenase and 12.5 pM Spirulina maxima Fd as electron mediator, (a) Conditions all components free (b) hydrogenase immobilized in Ca alginate according to Gisby and Hall (1980) (c) PSI particles immobilized in Ca alginate (d) hydrogenase and PSI coimmobilized in Ca alginate.

Redox schemes illustrating proposed action of Fe hydrogenases (A) Clostridium pasteurianum hydrogenase I (B) Clostridium pasteurianum hydrogenase... 

Fig. 12.1 Crystallographically characterized structures of Fe-only hydrogenases partially reduced hydrogenase of Desulfovihrio desuforicans (Ddht) (A), oxidized form of Clostridium pasteurianum hydrogenase I...

Trohalaki S, Pachter R (2010) The effects of the dimethylether bridging moiety in the h-duster of the Clostridium pasteurianum hydrogenase on the mechanism of h(2) production a quantum mechanics/molecular mechanics study. 

The [FeFe]-hydrogenases have been known since the 1930s [305], and Warburg recognized sulfur-coordinate iron as an essential element of the enzyme [306]. The [FeFe]-hydrogenase from Clostridium pasteurianum accommodates 20 iron atoms organized in one [2Fe-2S] cluster, three [4Fe S] clusters and the so-called H-cluster which is a [4Fe-4S] cluster covalently linked to a dinuclear [FeFe]... 

FeFe]-hydrogenase and models HRed. Clostridium pasteurianum 4.2 0.08 0.87 [310]... 

JW. Peters, WN. Lanzilotta, BJ. Lemon, LC. eefeldt (1998) X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 Angstrom resolution. Science, 282 1853-1858... 

Adams, M. W., Eccleston, E. and Howard, J. B. (1989) Iron-sulfur clusters of hydrogenase I and hydrogenase II of Clostridium pasteurianum. Proc. Natl. Acad. Sci. USA, 86, 4932-6. 

Erbes, D. L., Burris, R. H. and Orme-Johnson, W. H. (1975) On the iron-sulfur cluster in hydrogenase from Clostridium pasteurianum W5. Proc. Natl. Acad. Sci. USA, 72, 4795-9. 

Lemon, B. J. and Peters, J. W. (1999) Binding of exogenously added carbon monoxide at the active site of the iron-only hydrogenase (CpI) from Clostridium pasteurianum. Biochemistry, 38, 12969-73. 

Yanke LJ, Bryant RD, Laishley EJ. 1995. Hydrogenase of Clostridium pasteurianum functions as a novel selenite reductase. Anaerobe 1 61-7. 

We were also able to clearly demonstrate the Clostridium pasteurianum [Fe] hydrogenase 1 was a corrosive enzyme, accelerating the dessolution of iron and hydrogen production from mild steel surfaces (Bryant and Laishley 1990). We believe hydrogenases are intimately involved in the metal biocorrosion process. 

Many microbes contain a hydrogenase that lacks nickel. The best studied of these proteins is the hydrogenase from C. pasteurianum (Adams, 1990). The active site of this protein has been called the H cluster. Two structures of this protein recently appeared, a 1.6 structure of the het-erodimeric Desulfovibrio desuljuricans enzyme (Nicolet et al., 1999) and the 1.8 structure of the Cpl enzyme from Clostridium pasteurianum (Peters et al., 1998). The H cluster at the active site contains 6Fe as proposed earlier (Adams et al., 1989). There are two subcomponents a typical [4Fe64S] cubane bridged by a cysteine residue to an active site binuclear Fe center (Figure 4b). The unusual nature of the iron site in the NiFe hydrogenase is... 

Figure 1 (a) A schematic drawing of the active site of [NiFe] hydrogenases as deduced from the crystallographic studies. (h) A schematic drawing of the H-cluster of the CO-inhihited form of the Clostridium pasteurianum [Fe] hydrogenases as deduced from the recent X-ray crystallographic studies " ... 

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