Clostridium acetobutylicum, alcohol dehydrogenase

Young M, Minton NP, Staudenbauer WL (1989) Recent advances in the genetics of the Clostridia. FEMS Microbiol Rev 63 301-326 Youngleson JS, Santangelo JD, Jones DT, Woods DR (1988) Cloning and e3q>ression of a Clostridium acetobutylicum alcohol dehydrogenase gene in Escherichia coli. Appl Environ Microbiol 54 676-682... 

Alcohol dehydrogenase has been studied in several bacteria. Apart from work cited in Sund and Theorell (1), the following sources may be mentioned Clostridium acetobutylicum (481), Escherichia coli (482),... 

Green, E.M. and Bennett, G.N. (1996) Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. Appl. 

FIGURE 9.2 Physiology of ABE fermentation metabolism of Clostridium acetobutylicum with the respective enzymes and products. CoA, coenzyme A Ldh, lactate dehydrogenase Pdc, pyruvate decarboxylase Pfor, pyruvate ferredoxin oxidoreductase Fdred, ferredoxin reduced Thl, thiolase Hbd, p-hydroxybutyryl-CoA dehydrogenase Crt, crotonase Bed, butyryl-CoA dehydrogenase Etf, electron transfer flavoprotein Pta, phosphotransacetylase Ack, acetate kinase Ptb, phosphotransbutyrylase Buk, butyrate kinase Ctf A/B, acetoacetyl-CoA acyl-CoA transferase Adc, acetoacetate decarboxylase AdhE, aldehyde/alcohol dehydrogenase Bdh, butanol dehydrogenase. 

Dai Z, Dong H, Zhu Y, Zhang Y, Li Y, Ma Y. (2012). Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation. Biotechrwl Biofuels, 5,44. 

Tummala SB, Junne SG, Papoutsakis ET. (2003a). Antisense RNA downregulation of coenzyme a transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations. J Bacterial, 185, 3644-3653. 

Nair RV, Bennett GN, Papoutsakis ET (1994) Molecular characterization of an alcohol/aldehyde dehydrogenase gene of Clostridium acetobutylicum ATCC 824. J Bacteriol 176 871-885... 

Eischer CR, Klem-Marcuschamer D, Stephanopoulos G (2008) Selection and optimization of microbial hosts for biofuels production. Metab Eng 10(6) 295-304 Eontaine L et al (2002) Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol 184 (3) 821-30... 

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