Cloning and Expression of Pest Insect RyRs

virescens RyR. Sf9 cells expressing recombinant D. mdanogaster and H. virescens RyRs exhibit comparable Rynaxypyr sensitivity to that observed with native P. americana neurons. Expression of such recombinant insect RyRs offers utility for target screening and is the basis of a patent application [8]. 

Mammals possess three isoforms of the ryanodine receptor RyRl and RyR2, distributed primarily in skeletal and cardiac muscle, respectively, and RyR3 distributed more heterogeneously. Insects, however, express a single form of the receptor, sharing only 47% sequence homology [9]. Comparative studies were conducted to determine Rynaxypyr s ability to activate mammalian RyRs. 

Though ryanodine and caffeine show similar potency against insect and mammalian receptors (not shown), differential selectivity is observed for Rynaxypyr . 

Rynaxypyr is a highly potent and selective activator of insect RyRs. Activation of these receptors causes unregulated release of internal stores leading to store 

Miyata, T. Hosoya, I. Masai, Y. Hotta, FEBS Lett. 1994, 337, 81-87. 

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