Clone fingerprinting

In the human cell there are 23 pairs of chromosomes containing approximately 3000 million base pairs of DNA. Short sequences of DNA, perhaps with as few as 20 nucleotide units and sometimes radiolabeled, can be obtained either by chemical synthesis (gene machine) or from cloning. These short sequences can be used to probe for a complementary sequence by looking for the position to which they bind to any DNA sample under investigation, from blood for example. Such probes can detect as little as 100 fg of DNA and are the basis of forensic genetic fingerprinting tests. 

Fig. 5. In the experiment, carried out by Charles Weissmann and his co-workers, single Qp-RNA molecules (or viruses) from a wild-type distribution (a) were cloned in E. coli bacteria. After rapid multiplication the clones of individual RNA molecules (b) were analyzed and compared by the fingerprint method. Differences were noticed in one or two positions in the sequence. After a further, long period of reproduction (c) the wild-type distribution (d) was found in every clone, that is, the average sequences had become identical again.

Van den Ende, W., Michiels, A., De Roover, J., Verhaert, P., and Van Laere, A., Cloning and functional analysis of chicory root fructan 1-exohydrolase I (1-FEH I) a vacuolar enzyme derived from a cell-wall invertase ancestor Mass fingerprinting of the 1-FEH I enzyme, Plant J., 24, 447 156, 2000b. 

A knowledge of the restriction site used as the cloning site, and of possible restrictitm sites within the passenger DNA, is used to interpret the fingerprints. 

Gene technologies involve working with DNA and include DNA fingerprinting, cloning, and recombinant DNA. 

Determined by BslNI restriction fingerprinting that was carried out on part of the candidates for binders that were identified in the initial screen b Affinity was not determined for all the selected antibodies Clones isolated by the colony lift screen... 

Lander ES, Waterman MS (1988) Genomic mapping by fingerprinting random clones a mathematical analysis. Genomics 2 231-239... 

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