Classical Spectrophotometric Analysis, Limitations, and Early Attempts to Find a Solution

Classical Spectrophotometric Analysis, Limitations, and Early Attempts to Find a Solution 

The quantitative analysis of an isomolar mixture containing S absorbing species is based on the additivity principle, known as Beer s law  

When the individual spectra of the components are known (i.e., have been measured previously), the quantitative analysis can be routinely performed under given circumstances [5]. 

If a simplest two-component tautomeric equihbrium (b = a) is considered, Eq. (2.1) can be presented as 

The tautomeric constant Kj, defined according to Eq. (2.3) is used to describe the position of the equilibrium  

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