Ciprofloxacin liquid chromatographic methods

Roybal et al. (188) developed another liquid chromatographic method for the determination of enrofloxacin, ciprofloxacin, sarafloxacin, and difloxacin in milk. In this method, a 5 ml milk sample is extracted twice with a mixture of... 

The assay of an IV formulation is performed using a liquid chromatographic method, which requires the following solutions. For solution (1), dilute a quantity of the intravenous infusion with sufficient mobile phase to produce a solution containing the equivalent of 0.05% w/v of ciprofloxacin. Solution (2) contains 0.058% w/v of ciprofloxacin hydrochloride EPCRS in mobile phase. Solution (3) contains 0.025% w/v of ciprofloxacin impurity C EPCRS (ethylenediamine compound) in solution (2). For solution (4), dilute 1 volume of solution (3) to 100 volumes with the mobile phase. The chromatographic procedure may be carried out using a stainless steel column (12.5 cm x 4 mm) packed with stationary phase C (5 mm) (Nucleosil C18 is suitable). The mobile phase is eluted at a flow rate of 1.5 mL/min, and consists of a mixture of 13 volumes of acetonitrile and 87 volumes of a 0.245% w/v solution of orthophosphoric acid (the pH of which has been adjusted to 3.0 with triethylamine). Analytes are detected on the basis of their UV absorption at 278 nm. The assay is not valid unless in the chromatogram obtained with solution (3), the resolution factor between the peaks due to ciprofloxacin and the ciprofloxacin impurity C is at least 1.5. 

Torniainen, K. Maki, E. Development of an isocratic high-p>erformance liquid chromatographic method for monitoring of ciprofloxacin photodegradation. J.ChromatogrA., 1995, 697, 397-405... 

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