Cinchona alkaloids capillary

With capillary electrophoresis (CE), another modern primarily analytically oriented separation methodology has recently found its way into routine and research laboratories of the pharmaceutical industries. As the most beneficial characteristics over HPLC separations the extremely high efficiency leading to enhanced peak capacities and often better detectability of minor impurities, complementary selectivity profiles to HPLC due to a different separation mechanism as well as the capability to perform separations faster than by HPLC are frequently encountered as the most prominent advantages. On the negative side, there have to be mentioned detection sensitivity limitations due to the short path length of on-capillary UV detection, less robust methods, and occasionally problems with run-to-run repeatability. Nevertheless, CE assays have now been adopted by industrial labs as well and this holds in particular for enantiomer separations of chiral pharmaceuticals. While native cyclodextrins and their derivatives, respectively, are commonly employed as chiral additives to the BGEs to create mobility differences for the distinct enantiomers in the electric field, it could be demonstrated that cinchona alkaloids [128-130] and in particular their derivatives are applicable selectors for CE enantiomer separation of chiral acids [19,66,119,131-136]. 

Lammerhofer, M., Tobler, E., Zarbl, E., Lindner, W., Svec, E, and Frechet, J. M. J. (2003). Macroporous monolithic chiral stationary phases for capillary electrochromatography new chiral monomer derived from cinchona alkaloid with enhanced enantioselectivity. Electrophoresis 24, 2986-2999. 

Piette V, Fillet M, Lindner W, Crommen J (2000) Non-aqueous capillary electrophoretic enantioseparation of N-derivatized amino acids using cinchona alkaloids and derivatives as chiral counter-ions. J Chromatogr A 875 353-360... 

Tsimachidis D, cesla P, Hajek T, Theodoridis G, Jandera P. Capillary electrophoretic chiral separation of Cinchona alkaloids using a cyclodextrin selector. J. Sep. Sci. 2008 31 1130-1136. 

GLASS CAPILLARY GAS CHROMATOGRAPHY OF ALKALOIDS FROM A cinchona BARK PHARMACEUTICAL PREPARATION2. 

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