Chromium iodide analysis

Oxidation-reduction reactions can cause substantial errors in analysis. For example, soluble iron(II) and manganese(II) are oxidized to insoluble iron(III) and manganese(IV) compounds when an anaerobic water sample is exposed to atmospheric oxygen. Microbial activity can decrease phenol or biological ojg gen demand (BOD) values, change the nitrate-nitrite-ammonia balance, or alter the relative proportions of sulfate and sulfide. Iodide and cyanide frequently are oxidized. Chromium(VI) in solution may be reduced to insoluble chromium(III). Sodium, silicate, and boron are leached from glass container walls. 

Flow cytometry has been used successfully for analysis of apoptosis. Annexin V/Propidium iodide assay in this technique can differentiate between dead and live cells.This assay has been shown to provide a single-cell-based, rapid, quantitative, and sensitive assay to detect lymphocyte-mediated target cell killing in various animal models. Unlike conventional chromium release assays, the FC assay enables monitoring of cellular immune responses in real time and at the single-cell level using diverse fluorescence detection methods such as flow cytometry and fluorescence and CLSM [48]. 

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