Chromatography nickel-chelate

One limitation to this method should be noted. If the antibody-enzyme conjugate is prepared using antibody fragments such as Fab or F(ab )2, then nickel-chelate affinity chromatography will not work, since the requisite Fc portion of the antibody necessary for complexing with the metal is not present. 

Figure 20.15 An affinity chromatography support containing iminodiacetic acid groups chelated with nickel may be used to remove excess enzyme after reactions to produce antibody-enzyme conjugates. The nickel chelate binds to the antibody in the Fc region, retaining the conjugate while allowing free enzyme to pass through the gel unretarded.

Uden, P. C., Henderson, D. E., Burgett, C. A. Trace copper and nickel chelate detection by electron capture gas-chromatography. AnaL Lett. 7, 807 (1974)... 

Rogl, H., Kosemund, K., Kiihlbrandt, W., CoDinson, I. (1998) Refolding of Escherischia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography. FEES Letters, 432, 21—26. 

Preparation of sdV periplasmic extracts 87 Purification of scFv by nickel-chelate chromatography 88... 

For identification and purification, recombinant proteins are often tagged to the N-terminus with an additional sequence of histidyl residues, mostiysix (Hisg tag). This tag binds selectively to cations as nickel or copper immobilized by covalent chelators as nitrilotri-acetic acid. The method is named Metal Chelate Chromatography (MCC, MCAC, IMAC). 

Finally, it should be pointed out that until now the application of ion chromatography to the analysis of heavy and transition metals such as iron, copper, nickel, and manganese in the ppb range in 50% sodium hydroxide solution [96, 97] was not possible. A concentration procedure developed by Kingston et al. [98] is used for the AAS analysis of heavy metals in sea water. It is based on the selective concentration of heavy metals on a macroporous iminodiacetic acid resin, where divalent cations are retained by chelation. Their affinity toward the stationary phase decreases in the order... 

The trimethylsilylated silicic acids formed in this instance are soluble in conventional organic solvents, and their volatility is sufficiently high for them to be analysed by gas chromatography. Carzo and Hoebbel [411] carried out a comprehensive study of the chromatographic retention of various trimethylsilylated silicic acids on different stationary phases Apiezon L and silicone OV-1 and OV-17. The analysis of metals in the form of volatile complexes continues to attract attention, and have been described for analysing sodium [412], potassium [412], radium [413], caesium [413], barium [414], calcium [414], strontium [415], beryllium [416, 417], magnesium [418], zinc [419, 420], nickel [419], mercury [421], copper [422, 423], silver [424, 425], cadmium [421], indium [426, 427], g ium [428], scandium [217], cobalt [421], thallium [426], hafnium [429, 430], lead [431, 432], titanium [430], vanadium [433], chromium [434-436], manganese [426], iron [437], yttrium [438], platinum [439,440], palladium [439, 441, 442], zirconium [430], molybdenum [443], ruthenium [444], rhodium [445], rare earths [446—449], thorium [221, 450, 451] and uranium [221, 452]. The literature on GC analysis of metal chelates was reviewed by Sokolov [458]. 

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