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Chromatography hydrophobic interactions chromatograph

After initial cell fermentation and product extraction from the producer cells, the crude preparation is subject to multiple chromatographic steps, including ion-exchange, hydrophobic interaction chromatography and gel-filtration chromatography. The purified product is presented in lyophilized form in vials (1 mg active/vial) and excipients include a phosphate buffer, sodium chloride and serum albumin. [Pg.261]

In liquid chromatographic methods, reversed-phase columns are usually employed due to hydrophobic interaction of the amphenicols molecules witli the Cs or Ci8 stationary phases. Ion-pairing liquid chromatography has been also described for the separation of florfenicol and florfenicol amine (62) or chloramphenicol and deacetylchloramphenicol residues (46) using heptanesulfonate or octylsulfate-pairing ions, respectively. [Pg.900]

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See also in sourсe #XX -- [ Pg.321 ]



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Hydrophobic interaction chromatography

Hydrophobic interactions

Hydrophobic interactions chromatograph

Hydrophobic/hydrophobicity interactions

Hydrophobized interaction

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