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Chromatogram, absorption band

Chromatograms of the optical resolution of (I) and (II) Eire shown in Figure. 1. Compound (II) is completely resolved into its enantiomers chromatographically. The g-factor remains at its constant optimum value throughout the elution band of each enantiomer. Compound (I) in contrast is incompletely resolved, giving a single absorbance elution band, but a double bisignate difference absorbance band. The fractions of (I) eluted in the volume between the vertical dashed lines are incompletely resolved. Values for the g-max factors have been determined from the CD and absorption spectra of (I) and (II). [Pg.284]

The diode-array detector (DAD) enables the simultaneous measurement of absorption versus analysis time (chromatogram) and absorption versus wavelength (spectrum), without having to stop the mobile phase flow. Diode-array detectors can record spectra simultaneously every few milliseconds at different points of an elution band (peak) during a chromatographic run. Usually the spectra are taken at the beginning, the maximum, and the... [Pg.272]

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Absorption bands

Absorption chromatogram

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