Chromatium strain

The Chromatium transhydrogenase was isolated by Keister and Hemmes 16) from a sonicated extract of Chromatium strain D. After high-speed centrifugation and ammonium sulfate precipitation, two subsequent steps of DEAE-cellulose chromatography were employed. The purity of the preparation was tested for various reductase.and dehydrogenase activities. 

A particularly fine example of multiple recurrences of short as well as longer sequences, with standard interchanges and short-range rearrangements, is provided by the heme peptide isolated as a fragment from the heme protein of the photoanaerobe Chromatium (strain D) (Dus et al., 1961)... 

The most throughly studied ribulosebisphosphate carboxylase is from spinach, but carboxylases from many other species—including tobacco Chromatium strain D, a purple sulfur bacterium Chlorella ellvpsoidea, a green alga and Rhodospirillum a purple nonsulfur bac-... 

D-Arabinose occurs in arabinogalactans and arabinomannans elaborated by Mycobacterium species. When this had been determined, for example, for some arabinomannans, it was found to be furanosidic and a-linked. The arabinogalactan from Mycobacterium tuberculosis,however, contains both a- and y -linked D-arabinofuranosyl residues. It also occurs in the a-form in the LPS from Pseudomonas maltophila strain NCIB 9204. l-Arabinose is a component of the LPS from the purple, sulfur bacterium Chromatium vinosum. °... 

Type III-PHA synthase is represented by the enzyme of Chromatium vino-sum and is encoded by phaECCv. It consists of the two different subunits PhaCCv and PhaECv exhibiting molecular weights of 39,730 and 40,525 Da, respectively [23]. The native PHA synthase, as isolated from recombinant strains of E. coli, exhibited a molecular weight of approximately 390 and 400 + 20 kDa as revealed in our laboratory [54] or 360 + 50 kDa but also 520 + 50 kDa as revealed in another laboratory [55]. The lower molecular weights for the holoenzyme are... 

Fig. 8. BPR spectra of [3Fe-xS] clusters in oxidized hydrogenases, showing th influences of weak Ni-Fe-S electron-spin interactions, (a) Desulfovibrio desulfurican (strain Norway 4) hydrogenase, showing the spectrum of an isolated [3Fe-xS] cluster (b Chromatium vinosum hydrogenase the outer lines (Signal 2) correspond to interactio with Ni(lH) (c) Paracoccus denitrificans hydrogenase (d) Alcaligenes eutrophu membrane-bound hydrogenase. Spectra were recorded at approximately 20 K. Sample were provided by K. K. Rao, J. Serra, and K. Schneider.

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