Chorismate engineered

Within the diastereomeric switch sequences, the corresponding trans-diols become accessible either using a Mitsunobu inversion or a reversible Diels-Alder cyclization as key reaction step [249,250]. This synthetic strategy is complementary to an approach involving metabolic engineering of E. coli via the chorismate/ isochorismate pathway [251]. 

Over the past decade, several strains of yeast [43, 44] and E. coli [45, 46] have been engineered that lack chorismate mutase. A typical bacterial selection system is depicted schematically in Fig. 3.5. It is based on E. coli strain KA12 [45], which has deletions of the chromosomal genes for both bifunctional chorismate mutases (chorismate mutase-prephenate dehydrogenase and chorismate mutase-prephenate dehydratase). Monofunctional versions of prephenate dehydratase [47] and prephenate dehydrogenase [48] from other organisms are supplied by the plasmid pKIMP-UAUC, leaving the cells deficient only in chorismate mutase activity [45]. 

Fig. 3.5. An engineered E. coli selection system lacking endogenous chorismate mutase activity [45]. The genes encoding the bifunctional enzymes chorismate mutase-prephenate dehydrogenase and chorismate mutase-prephenate dehydratase were deleted, and monofunctional versions of the dehydrogenase and dehydratase were supplied on plasmid pKIMP-UAUC. Poten-...

Sprenger, G.A. (2007) From scratch to value engineering Escherichia coli wild type cells to the production of L-phenylalanine and other fine chemicals derived from chorismate. Appl. Microbiol Biotechnol., 75, 739-749. 

Chavez-Bejar, M.l. et al (2008) Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas... 

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