Cholesteryl quantitative determination

The solvent chloroform-methanol-water (65 + 25 + 4), described by Wagner et al. [175] has foimd extensive apphcation in the thin-layer chromatographic separation of serum phospholipids on silica gel 6 layers. Habebmann and co-workers [67] have published in this connection a method for quantitative determination of the single phospholipid fractions. The methods so far known for separating phospholipids fractionate them only into groups of compounds this contrasts with the TLC of cholesteryl esters, where separation into individual substances is possible. The results hitherto obtained are summarised in Table 124. 

A procedure for determination of lipid hydroperoxides in human plasma is based on kinetic measurement of the CL of luminol (124) with hemin (75a) catalysis . CLD of microperoxidase-catalyzed oxidation of luminol (124) or isoluminol (190) was applied to detection and determination of amino acid hydroperoxides after exposure to UV and y-irradiation A method for determination of hydroperoxides in the phospholipids of cultured cells uses isoluminol (190) and microperoxidase as catalyst " . Simultaneous determination of phosphatidylcholine hydroperoxides and cholesteryl ester hydroperoxides in human serum is carried out by quantitative extraction of the lipids, HPLC separation by column switching and CLD using isoluminol (190) with microperoxidase catalysis . ... 

Quantitative infrared spectroscopic analysis can be carried out on blood serum to determine the relative amounts of lipid that are present. Triglycerides, phospholipids and cholesteryl esters are the classes of lipid which occur in blood serum. These compounds occur naturally in concentrations which make infrared analysis attractive, and the necessary preliminary separation is simple. These classes of compounds can be characterised using infrared spectroscopy by their carbonyl bands. The peak maxima are as follows ... 

The oxidation products of plasma and lipoprotein phospholipids have been the subject of extensive study by chromatographic and mass spectrometric methods. Earlier, workers had reported the simultaneous determination of phospholipid hydroperoxides and cholesteryl ester hydroperoxides in human plasma by HPLC with chemiluminescence monitoring. Lipid hydroperoxides were quantitatively extracted from human plasma with a mixture of -hexane and... 

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