Chloroform fumigation-incubation method

Historically, measurement of the microbial biomass has been a tedious, time-consuming occupation involving staining and direct counting or use of culture media and enumeration of individual microbial communities. However, in the last 20 years, a suite of methods have been developed for more rapid assessment of the microbial biomass. These include the substrate-induced respiration method (Anderson and Domsch 1978), the chloroform fumigation-incubation method (Jenkinson and... 

Voroney, R.P., and E.A Paul, (1984), Determination of K and in situ for Calibration of the Chloroform Fumigation Incubation Method. Soil Biol. Biochem. 16 9-14. 

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