Chloroform fumigation-incubation

Historically, measurement of the microbial biomass has been a tedious, time-consuming occupation involving staining and direct counting or use of culture media and enumeration of individual microbial communities. However, in the last 20 years, a suite of methods have been developed for more rapid assessment of the microbial biomass. These include the substrate-induced respiration method (Anderson and Domsch 1978), the chloroform fumigation-incubation method (Jenkinson and... 

Microbial biomass carbon Chloroform fumigation incubation Indicates soil microbial population various methodologies controls nutrient cycling and biological transformation necessary for soil aggregation dependent upon organic inputs Rice et al. (1996)... 

Voroney, R.P., and E.A Paul, (1984), Determination of K and in situ for Calibration of the Chloroform Fumigation Incubation Method. Soil Biol. Biochem. 16 9-14. 

Incubated under sterile (chloroform fumigation) and nonsterile conditions (organic acid application according to the composition of root exudates). 

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