Chloroform extract anti-inflammatory activity

In another paper, Rios et al. [10] reported the anti-inflammatory activity of the chloroform extract from Cayaponia tayuya, which exhibited a high potency against carrageenan-induced mouse paw edema, with an ED50 of 122.5 mg/kg (p.o.) and 27.8 (i.p.). From the active extract, Recio et al. [43] isolated two cucurbitacins which were identified as cucurbitacin R and 23,24-dihydrocucurbitacin B, Fig. (7). 

Some active species have been studied and their pharmacological activity confirmed in different experimental models. In general, the active fractions of such plants were those containing cucurbitacins, as demonstrated by Rios et al. [10] in their work with Cayaponia tayuya roots. This species is used in the Amazon region of South America as an analgesic, anti-inflammatory, and anti-rheumatic agent while the extracts of this species are widely employed in the treatment of skin disorders such as dermatosis and other irritations. The chloroform fraction obtained from the active root methanol extract was found to be more active than the ethyl acetate and butanol fractions. Subsequent phytochemical analysis demonstrated that the chloroform fraction is rich in cucurbitacins, whereas the ethyl acetate and butanol fractions are principally comprised of C-glycosil flavonoids [10]. 

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