Chlamydomonas reinhardtii, hydrogen

Chiral sulfides, dioxirane oxidation, 1156-7 Chlamydomonas reinhardtii, hydrogen peroxide determination, 646 y-Chlordene, isomers, 728 Chlorellajusca, hydrogen peroxide determination, 646... 

PS1 The PS 1-prep, introduced in this communication is the first reported with a polyhistidine tag fused to the N-terminus of the PsaF subunit. This construct was possible due to the fact that cyanobacterial PsaF-deletion mutants show no impact on photoautotrophic growth - in contrast to Chlamydomonas reinhardtii, where inactivation of PsaF results in a severe reduction of electron transfer from plastocyanin to PS 1 [Hippier et al. 1997], Also, the N-terminus of the F-subunit which was decorated by the tag is located towards the lumen side which enables an attachment of the isolated PS1 with the lumen-exposed /donor-side to the electrode surface in our hydrogen-producing device. 

Melis, A., Zhang, L., Forestier, M., Ghirardi, M. L. and Seibert, M. (2000) Sustained photo-biological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol., 122, 127-36. 

Tsygankov, A.A., Kosourov, S.N., Tolstygina, I.V., Ghirardi, M.L., Seibert, M. 2006. Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautrophic conditions. Int J Hydrogen Energy 31 1574-1585. 

Kosourov S, Tsygankov A, Seibert M, Ghirardi ML (2002) Sustained hydrogen photoproduction by Chlamydomonas reinhardtii. effects of culture parameters. Biotechnol Bioeng 78 731-740... 

Witt, H., Schlodder, E., Teutloff, C., Niklas, J. Bordignon, E., Carbonera, D., Kohler, S., Labahn, A., and Lubitz, W. (2002) Hydrogen bonding to P700 site-directed mutagenesis of threonine A739 of Photosystem I in Chlamydomonas reinhardtii, Biochemistry 41, 8557-8569... 

Amos, W. 2004. Updated Cost Analysis of Photobiological Hydrogen Production from Chlamydomonas reinhardtii Green Algae. NREL/MP-560-35593. http //www.nrel.gov/docs /fy04osti/35593.pdf. 

Fedorov, A.S., Kosourov, S., Ghirardi, M.L. and Seibert, M. 2005. Continuous hydrogen photoproduction by Chlamydomonas reinhardtii using a novel two-stage, sulfate-limited chemostat system. Appl. Biochem. Biotechnol., 121-124, 403-412. 

Laurinavichene, T., Tolstyginina, I. and Tsygankov, A. 2004. The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii. J. Biotechnol. 114, 143-151. 

Posewitz, M.C., Smolinski, S.L, Kanakagiri, S., Melis, A., Seibert, M. and Ghirardi, M.L. 2004b. Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16, 2151-2163. 

Laurinavichene, T.V. et al.. Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells, Int. J. Hydrogen Energy, 31, 659, 2006. 

Dante, R. (2004). Hypotheses for direct PEM fuel cells applications of photobiopro-duced hydrogen by Chlamydomonas reinhardtii. Int.. Hydrogen Energy (in press). 

Tsygankov, A., Kosourov, S., Seibert, M., Ghirardi, M. (2002b). Hydrogen photoproduction under continuous illumination by sulphus-deprived, synchronous Chlamydomonas reinhardtii cultures. Int. ]. Hydrogen Energy 27,1239-1244. 

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