Chitosans hyaluronic acid complexes

Nath, S.D., Abueva, C., Kim, B., Lee, B.T., 2015. Chitosan-hyaluronic acid polyelectrolyte complex scaffold crossbnked with genipin for immobilization and controlled release of BMP-2. Carbohydr. Polym. 115, 160-169. 

Polyelectrolyte complexes composed of various weight ratios of chitosan and hyaluronic acid were found to swell rapidly, reaching equilibrium within 30 min, and exhibited relatively high swelling ratios of 250-325% at room temperature. The swelling ratio increased when the pH of the buffer was below pH 6, as a result of the dissociation of the ionic bonds, and with increments of temperature. Therefore, the swelling ratios of the films were pH-and temperature-dependent. The amount of free water in the complex films increased with increasing chitosan content up to 64% free water, with an additional bound-water content of over 12% [29]. 

The optimum conditions for polyion complex formation between chitosan and hyaluronate were identified fhe compression exerfed fo manufacture the implant had no role to play in the release kinetics [28,92]. Various authors published data confirming fhaf fhe combinafion of chifosan and hyaluronic acid is always suscepfible fo swelling, even in fhe presence of cross-hnking. 

Heparin (as the cetylpyridinium salt) has been immobilized by reaction of its residual peptide chain with agarose cyclic imidocarbonate the immobilized mucopolysaccharide was used in separating two forms of a-L-iduronidase. Heparin reacted with chitosan to give a novel polyelectrolyte complex. Hyaluronic acid can be labelled with fluorescein, with only slight degradation, to give a derivative of d.s. 0.001—0.05. ... 

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