Chiral-phase chromatography, lipid analysis

Also extracted from native HZ were polar hydroxylated fatty acids derived presumably from cellular arachidonic and linoleic acids. Native HZ was purified from infected RBCs by a series of centrifugation steps followed by organic extraction of its lipid coat. Analysis of the lipid coat revealed the presence of hydroxylated polyunsaturated fatty acids. These polar lipids were separated using reverse phase-, normal phase-, and chiral phase high performance liquid chromatography and subsequently examined by GC-MS. Native FIZ lipid coat GC-MS analysis revealed the presence of 15-, 12-, 11-, 9-, 8-, and 5-hydroxyeicosatetraenoic acids (HETEs) as well as 13- and 9-hydroxyoctadecadienoic acids (HODEs) (35). 

In addition to normal- and reversed-phase chromatography, silver ion HPLC, RP ion-pair HPLC, chiral separation, and supercritical fluid chromatography have been used for analysis of lipids [3]. In silver-ion HPLC, the counterion of an ion-exchange column such as sulfonate is exchanged with silver ions. Only the degree of unsaturation in the lipid molecule determines retention. In RP ion-pair HPLC, different ion-pairing agents, such... 

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