Chip-type electrochemical flow

T.K. Lim, H. Ohta, and T. Matsunaga, Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples. Anal. Chem. 75, 3316-3321 (2003). 

M. Okochi, H. Ohta, T. Tanaka, and T. Matsunaga, Electrochemical probe for on-chip type flow immunoassay immunoglobulin G labeled with ferrocenecarboaldehyde. Biotechnol. Bioeng. 90, 14-19 (2005). 

Miniaturized immunosensors, which combine the analytical power of nuCTofluidic devices with the high specificity of antibody-antigen interactions, have been intensively developed [9-11, 47-51]. These platforms have proven to be highly suitable vehicles for conducting various immunoassay protocols. Our research groups have described a new approach to the performance of miniaturized electrochemical flow immunoassay system (on-chip typed flow immunoassay system) by using ferrocene-conjugated... 

Fig. 11.5 Electrochemical detection of immunocomplexes using on-chip typed cation-exchange chromatography and ferrocene-conjugated antibody. Flow rate 200 pl/min Column volume 30 pi, Buffer change point 350, 650 s...

Some support structures are also included for detachably retaining the various components of the system. Preferably the support structure can be of the assembly board type , which provides prearranged flow channels and connector ports. The desired components of the system can be fastened into these connectors by pins. The flow control system that makes up the ICS system can include pumps, flow channels, manifolds, flow restrictors, valves, etc. These components are equipped with the necessary fittings that allow them to be sealed with the prearranged or selectively located flow channels or connectors. The flow system can also include detachable mixing devices, e.g., static or ultrasonic, or with a chip-like design. The reaction units, whether chip-like or not, can be of thermal, electrochemical, photochemical or pressure type [84]. 

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