Chip Electrochromatography

(e) 11.4 and (f) 11.1 mm. The effective length of the main channel from the cross to point of detection 2 is approximately 25 mm. The channel depth is 9 tm and its width at half-depth is 50 xm. Reprinted from [82] with permission. 

---

Fintschenko, Y., Choi, W.Y., Ngola, S.M., Chip electrochromatography of polycyclic aromatic hydrocarbons on an acrylate-based UV-initiated porous polymer monolith, Fres. J. Anal. Chem. 2001, 371(2), 174-181. 

Jindal, R. and Cramer, S.M., On-chip electrochromatography using sol-gel immobilized stationary phase with UV absorbance detection, Journal of Chromatography A, 1044, 277-285, 2004. 

Lazar et al. " have recently demonstrated the first example of interfacing chip electrochromatography with mass spectrometric detection. The glass microchip contained a methacrylate-based monolith as well as an integrated capillary tip for ESI. The straight 130-p.m wide and 50-p.m deep main channel of the chip contained a 5 to 6-cm long separation section that led directly to the ESI... 

Faure K, Albert M, Dugas V, CretiCT G, Ferrigno R, Morin P, Rocca JL (2008) Development of an acrylate monolith in a cyclo-olefin copolymer microfluidic device for chip electrochromatography separation. Electrophoresis 29 4948-4955... 

Oleschuk, R. D., L. L. Shultz-Lockyear, Y. Ning, and D. J. Harrison. Trapping of bead-based reagents within microfluidic systems On-chip solid-phase extraction and electrochromatography. Anal. Chem. 72, 585-590 (2000). 

Ceriotti, L., N. F. de Rooij, and E. Verpoorte. An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases. Anal. Chem. 74, 639-647 (2002). 

M. Galloway and S.A. Soper, Contact conductivity detection of polymerase chain reaction products analyzed by reverse-phase ion pair microcapillary electrochromatography, Electrophoresis, 23 (2002) 3760-3768. M. Masar, M. Dankova, E. Olvecka, A. Stachurova, D. Kaniansky and B. Stanislawski, Determination of free sulfite in wine by zone electrophoresis with isotachophoresis sample pretreatment on a column-coupling chip, J. Chromatogr. A, 1026 (2004) 31-39. 

Electrochromatography was first demonstrated in the open-tubular microfluidic channel coated with octadecylsilane (ODS) as the chromatographic stationary phase. EOF was used as the pumping system within the 5.6-pm-deep and 66-pm-wide channels. Plate heights of 5.0—44.8 pm were achieved for three cou-marin dyes [112]. Open-channel CEC separation was achieved using a stationary phase of octadecyltrimethoxysilane [148], CEC separation of some PAHs has also been carried out on a Pyrex chip with a thin film of C8 stationary phase formed using a sol-gel process [338],... 

Constantin, S., Freitag, R., Solignac, D., Sayah, A., Gijs, M.A.M., Utilization of the sol-gel technique for the development of novel stationary phases for capillary electrochromatography on a chip. Sensors Actuators B 2001, 78, 267-272. 

Finot, M., Jemere, A.B., Oleschuk, R.D., Takahashi, L., Harrison, D.J., High throughput pharmaceutical formulation evaluation and analysis using capillary electrochromatography on a microfluidic chip. Micro Total Analysis Systems, Proceedings 5th lTAS Symposium, Monterey, CA, Oct. 21-25, 2001, 480-482. 

Slentz, B.E., Penner, N.A., Regnier, F.E., Capillary electrochromatography of peptides on microfabricated poly(dimethylsiloxane) chips modified by cerium(IV)-catalyzed polymerization. J. Chromatogr. A 2002, 948, 225-233. 

---