Chimeric/hybrid gene

Chromosomal translocation that fuses two genes together to produce a hybrid gene encoding a chimeric... 

Volume 326. Applications of Chimeric Genes and Hybrid Proteins (Part A Gene Expression and Protein Purification)... 

The presence of the chimeric construct in each ars-transformant was verified by PCR using an upstream oligonucleotide that hybridizes to the hydA promoter fragment and a downstream oligonucleotide that hybridizes to the ars gene asindicated with black arrows at the bottom. 

These conditions are associated with high renin and high aldosterone production. The hybrid corticosteroids 18-OHF, 18-oxo-F and 18-oxo-THF were isolated from the urine of a patient with adrenal adenoma by Ulick and coworkers [11]. These steroids were subsequently also shown to be dominant in GRA. Their formation in the latter condition was explained by Lifton and co-workers [51] with the discovery of a chimeric gene formed crossover of the 1 1//-hydroxylase (CYPllfil) and aldosterone synthase (CYP11B2) genes. 

Figure 7.5 Example of a chimeric oligonucleic acid and its modification. Chimeric RNA-DNA hybrids are used for correction of point mutations in target genes. One strand of this oligonucleic acid is composed of O-methyl-RNA (outline) with an interruption of 5 bases of deoxyribonucleic acid. X and Y are target residues for correction. In the complementary strand, there is a DNA nick, and T residues loop both ends. 3 -exonuclease and FEN-1 may act on the nick, PARP-1 possibly binds to and is activated by the nick, resulting in activation of damage response pathways. In the modified version, the 3 end is replaced by ribonucleic acids. The 5 end is extended, and the flipped back RNA tail is added. Thus, the nick is expected to be resistant to 3 -exonuclease and FEN-1. In addition, PARP-1 may not be activated by such a nick.

Example of a chimeric oligonucleic acid and its modification. Chimeric RNA—DNA 119 hybrids are used for correction of point mutations in target genes... 

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