Chemical synthesis glycosylation methods

Several chemical and enzymatic methods are available for the synthesis of glycosyl phosphates. The required nucleoside triphosphates (NTPs) are most conveniently prepared by enzymatic routes. In general, these methods involve the sequential use of two kinases to transform NMPs to NTPs, by the corresponding NDPs (Fig. 2, see a recent review [12] for more details). 

The combined use of enzymatic and chemical methods under the so-called chemoenzymatic synthesis offers the flexibility of chemical synthesis and the high regioselectivity and stereoselectivity of enzyme-catalyzed reactions to achieve highly efficient synthesis of complex carbohydrates. Chemoenzymatic glycosylation is... 

The problematic chemical synthesis of the ergot alkaloid glycosides prompted us to test enzymatic glycosylation methods. 

In recent years, tremendous progress has been made in the chemical synthesis of oligosaccharides. New glycosylation and protection methods resulted in new strategies and techniques for the preparation of oligosaccharides. As a result, large and complex oligosaccharides... 

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