Chemical genetics conditional mutations

The hybridisation event is affected by the concentration of DNA target, concentration and size of capture probe, temperature, hybridisation time and hybridisation buffer composition (pH, ionic strength, denaturalising chemical agents, etc.). The control of the experimental variables that affect the hybridisation event is very important in order to obtain an efficient and selective hybridisation. The detection of SNP or genetic mutations requires an efficient discrimination between mismatched and complementary strands. In most of the cases, the selectivity relies on the operating conditions of the assay as hybridisation buffer composition, hybridisation time and hybridisation temperature. 

Xenobiotics, for the most part, have limited biological half-lives. The extent of their influence on the biological living system is limited on the basis of the amount absorbed and the duration of their presence within the system. Once exposure to a pesticide chemical occurs, individuals need not be unduly fearful of adverse health impacts slowly and relentlessly progressing regardless of dose and duration of exposure. There are effects such as cancer induction and genetic mutations which have yet to be characterized and fully understood from a chemical standpoint. Consideration of these special conditions has been the subject of entire seminars and obviously cannot be covered fully in a presentation such as this. 

There is an opinion, that mechanisms of adverse effects on fetus development include transmission of chemical substances from the father to an embryo through a seed liquid, so-called pass-through "infection" of mother to an embryo - the substance brought by the man from a workplace or in domestic conditions. Besides, harmful chemical substances at man s workplace can induce genetic mutations. 

---