Chelation cell permeability increased

The addition of heparin to the isolated rat diaphragm previously loaded with rubidium 86 results in change of the inflow of rubidium . Heparin has a permeabilizing action which appears to be due to mobilization of bound potassium and this can be related to a reduction of calcium in the perfusion solution. Karasek and Mourek.i conclude that heparin depresses oxidative processes through an effect on cell permeability it is possible that it adsorbs certain substances such as potassium chloride or acetylcholine. In extracorporeal dialysis , heparin causes an apparent decrease of 60 per cent in plasma calcium concentration. Heparin also forms a chelate-like compound with calcium ions " . This process is usually unimportant as heparin absorbs a maximum of only 5 per cent of its weight of calcium, but with intradermal, intramuscular or subcutaneous administration, heparin may deplete the capillary walls of calcium and cause them to become fragile. Heparin alsc decreases wound strength 6-14 days after operation in some experiments and may delay the union of fractures in bone repair . Heparin, but not chondroitin sulphate or hyaluronic acid, in tissue culture increases the amount of bone resorption in the presence of suboptimal concentrations of parathyroid extracts, and thus may be a cofactor in bone metabolism. ... 

Penetration enhancers have been used to facilitate the absorption of higher molecular weight molecules. The mode of action of the surfactant enhancers is often attributed to membrane damage [37]. However, studies in epithelial cell monolayers suggest that some surfactant-based absorption enhancers act primarily by increasing the permeability of tight junctions [38]. Nevertheless, except for the chelators and nonsurfactants, which exert their... 

Penetration enhancers act by increasing the permeability of the corneal cell membrane and/or loosening the tight junctions between the epithelial cells, which primarily restrict the entry of molecules via the paracellular pathway. Classes of penetration enhancers include surfactants, bile salts, calcium chelators, preservatives, fatty acids, and some glycosides such a saponin. 

Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that binds certain metals, especially iron and copper, which are essential to the nutrition of certain microorganisms. In this manner, it is a strong booster or enhancer of the activity of preservatives especially the parabens. Alone, it has the ability to increase the permeability of the bacterial cell wall and can kill Pseudomonas aeruginosa and E. coli by this activity effective concentration is 0.05-0.10%. 

Several mechanisms have been proposed to explain the antimicrobial properties of tannins. The antimicrobial effects of phenolic compounds are probably related to the inhibition of bacterial enzymes, alterations in cell wall permeability, an increase in the hydrogen ion activity of the microbial environment, a reduction in the surface and/or interfacial tension and perhaps chelation of essential minerals, particularly iron with a concomitant impairment of the microbial oxidative metabolic system (Chung et al. 1998). The antimicrobial activities of tannins are ascribed to the interactions of goats tannins with the extracellular enzymes secreted and... 

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