Characterization of binding site

Simon EJ, Gioannini TL. Opioid receptor multiplicity isolation, purification and chemical characterization of binding sites. In Opioids I. Handbook of Experimental Pharmacology. Vol. 104 (Herz A, ed). Springer, Heidelberg, 1993 3-26. 

Garcia-Calzon JA, Diaz-Garcia ME. Characterization of binding sites in molecularly imprinted polymers. Sens ActuatB 2007 123 1180-1194. 

Du LP, Li MY, Tsai KC, You QD, Xia L. Characterization of binding site of closed-state KCNQ1 potassium channel by homology modeling, molecular docking, and pharmacophore identification. Biochem Biophys Res Commun 2005 332 677-87. 

Araujo, D. M. and Cotman, C. W., Basic FGF in astroglial, microglial, and neuronal cultures. Characterization of binding sites and modulation of release by lymphokines and trophic factors, J. Neurosci., 112, 1668, 1992. 

Boulanger, Y. Schreier, S. Leitch, L. C. Smith, I. C. R, Multiple binding sites for local anesthetics in membranes Characterization of the sites and their equilibria by deuterium NMR of specifically deuterated procaine and tetracaine, Can. J. Biochem. 58, 986-995 (1980). 

Carrilho, E. N. V. M., Ferreira, A. G. and Gilbert, T. R. (2002). Characterization of sorption sites on Pilayella littoralis and metal binding assessment using 113Cd and 27A1 nuclear magnetic resonance, Environ. Sci. Technol., 36, 2003-2007. 

The law of mass action describes the hyperbolic relationship between binding (B) and ligand concentration (c). This relationship is characterized by the drug s affinity (1/Itotal number of binding sites per unit of weight of membrane homogenate. 

Reiss, Y., Kaim, D., and Hershko, A. (1988). Specificity of binding of NH2-terminal residue of proteins to ubiquitin-protein ligase Use of amino acid derivatives to characterize specific binding sites. J. Biol. Chem. 263, 2693-2698. 

Merging both equations and transformation of the result gives the Scatchard graph, characterized by plotting [B]/[F] on ordinate and 1/Kd on abscissa. The constant Bmax represents that concentration of L needed for complete saturation of all binding sites at the receptor and the maximal number of binding sites, respectively. 

Cacho, C., E. Turiel, A. Martin-Esteban, C. Perez-Conde, and C. Camara (2004). Characterization and quality assessment of binding sites on a propazine-imprinted polymer prepared by precipitation polymerization. J. Chromatogr. B, 802 347-353. 

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