Scatchard graphs

Klotz IM. 1982. Numbers of receptor sites from Scatchard graphs facts and fantasies. Science 217 1247. 

Merging both equations and transformation of the result gives the Scatchard graph, characterized by plotting [B]/[F] on ordinate and 1/Kd on abscissa. The constant Bmax represents that concentration of L needed for complete saturation of all binding sites at the receptor and the maximal number of binding sites, respectively. 

G. Sposito, On the use of the Langmuir equation in the interpretation of adsorption phenomena. II The two-surface Langmuir equation. Soil Sci. Soc. Am. J. 46 1147 (1982). See also I. M. Klotz, Numbers of receptor sites from Scatchard graphs Facts and fantasies. Science 217 1247 (1982). (In the literature of macromolecular chemistry, Fig. 4.2 is known as a Scatchard plot.)... 

Munson, P. J. and Rodbard, D. (1983). Number of receptor sites from Scatchard and Klotz graphs a constructive critique, Science, 220, 979-981. 

A graph of [PX]/[X] versus [PX] has a slope of —K and is called a Scatchard plot.5 It is widely used in biochemistry to measure equilibrium constants (Figure 19-7). 

C. The appropriate Scatchard plot is a graph of AA/[X versus AA (Equation 19-16). 

Each reaction mixture provides data for one point on the binding graph. Collect data from other students to complete the graph. What are the shapes of curve 1 and curve 2 Does the Scatchard plot show cooperativity ... 

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