CHAPS dimethylammonio -1 -propane

Champeil, MoUer, 2000). Some lipid/detergent mixtures have been used in nuclear magnetic resonance (NMR) and crystallization experiments (Seddon, Cumow, Booth, 2004) in structural and functional studies of the extremely light-sensitive G protein-coupled receptor (GPCR), rhodopsin, Reeves et al. used a mixture of the lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine and the detergent 3-[(3-cholamidopropyl)-dimethylammonio]- -propane-sulfonate (CHAPS) (Reeves, Hwa, Khorana, 1999). 

Because HIC is based on surface-tension phenomena, changing those characteristics by the addition of surfactants affects retention. In a study of the effects of surfactants on retention of proteins by HIC, the addition of CHAPS 3-[(3-cholamidopropyl) dimethylammonio]-1-propane sulfonate to the mobile phase resulted in shortened retention, improvement of peak shape, and a change in peak order for enolase and bovine pancreatic trypsin inhibitor.The effects were dependent on the concentration of the surfactant. Surfactants can usually be washed easily from hydrophobic interaction columns because the bonded phases are neither highly hydrophobic nor ionic. 

CHAPS. See 3-[(3-Cholamidopropyl) dimethylammonio]-1-propane ulfonate Chimyl alcohol... 

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