Cerebrospinal fluid thiamin

The normal values for thiamine in human blood vary from 25-80 mpg/ml (average of 27 cases), from 110-370 mfig/ml in urine (27 cases), and from 13-17 mpg/ml in cerebrospinal fluid (45 cases). These specimens were obtained from normal subjects, receiving no vitamin therapy and in the fasting state, to eliminate dietary influences. The... 

Botez MI, Joyal C, Maag U, Bachevalier J. Cerebrospinal fluid and blood thiamine concentrations in phenytoin-trea-ted epileptics. Can J Neurol Sci 1982 9(l) 37-9. 

Both thiamin monophosphate and free thiamin are found in cerebrospinal fluid. Uptake of thiamin monophosphate into cells in the central nervous system involves extracellular hydrolysis to free thiamin, probably catalyzed... 

TPP and TTP occur in the central nervous system and play an important part in brain metabolism. Entry of thiamine into cerebrospinal fluid may occur via a saturable transport mechanism, perhaps located in the choroid plexus. 

Fig. 11.8.4. HPLC of thiamine in human serum (a) and human cerebrospinal fluid (b). Chromatographic conditions stationary phase, pBondapak C18 (10 pm) reversed phase (300 X 3.9 mm I.D.) mobile phase, methanol-aqueous sodium citrate, pH 4.0, 0.05 mol/1 (45/55, v/v), sodium 1-octanesulphonate 10 mmol/1 temperature, ambient flow rate, 1.2 ml/min detection, post-column fluorescence (excitation at 367 nm, emission at 435 nm). Peaks 1, saUcylamide 2, thiamine. Reproduced from Wielders and Mink (1983), with permission.

Jimenez-Jimenez, F. J., et al., 1999. Cerebrospinal fluid levels of thiamine in patients with Parkinson s disease. Neurosci Lett. 271, 33-36. 

Jimenez-Jimenez, F.J., Molina, J.A., Hernanz, A., Fernandez-Vivancos, E., de Bustos, F., Barcenilla, B., Gomez-Escalonilla, C., Zurdo, M., Berbel, A., and Villanueva, C., 1999. Cerebrospinal fluid levels of thiamine in patients with Parkinson s disease. Neuroscience Letters. 271 33-36. 

A similar HPLC method with a postcolumn derivatization system was used for the analysis of total thiamine in human whole blood as well as in serum, cerebrospinal fluid, and milk (26). The HPLC system consisted of a pBondapak column and the mobile phase was a mixture of methanol-50 mM sodium citrate buffer pH 4.0 (45 55, v/v) plus 10 mM sodium 1-octanesulfonate. Two milliliters of blood was needed. The minimum detectable amount was 60 fmol of thiamine. The intra-assay and inter-assay coefficients of variation were 2.3% and 3.9%, respectively. The recovery of TPP added to blood samples was 98.7%. 

JPM Wielders, CJK Mink. Quantitative analysis of total thiamine in human blood, milk and cerebrospinal fluid by reversed-phase ion-pair high-performance liquid chromatography. J Chromatogr 277 145-156, 1983. 

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