Cerebrosides, identification

Figure 7. Silica HPLC of product from galactose oxidase-treated myelin which were "coated by cerebrosides. See caption to Figure 4 for peak identification.

Fischer, G., and Jatzkewitz, H., The activator of cerebroside sulphatase Purification from human liver and identification as a protein. Hoppe-Seyler s Z. Physiol. Chem. 356, 605-613 (1975). 

Separation and Identification of Cerebrosides in Cerebrospinal Fluid by Gas Chromatography-Mass Spectrometry... 

With a better idea of how to identify possible isobars at a given m/z value, a better characterization of the ions detected can be accomplished. The identification of the PLs for the tissue section analyzed is described in Table 12.3. As can be seen, there is a DHB cluster ion detected at every mass isolated (typically arising from a neutral loss of 137, 154, 155, or 176). Many of the ions isolated for tandem MS show three and even four lipid species. The three primary lipid classes detected in the positive ion mode were PC, PS, and PE. Cerebrosides were also detected, but were present at a much lower intensity due to the conditions employed (18). The primary reason for maintaining an isolation width of 1.5 for most experiments was that PLs are considered fragile ions in ion trap analysis (26, 31) and thus can be lost during isolation if the isolation width is not wide enough. 

The sulfatide isolated was soluble in chloroform-methanol (2—1) and failed to partition into an aqueous phase. Further identification of the sulfolipid was not made. Goldberg (1961) has postulated several pathways which may be involved in cerebroside sulfate synthesis. The initial steps are the activations of sulfate to 3 -phosphoadenosine-5 -phosphosulfate, i.e., PAPS, as described by Bandurski et al. (1956, 1958), Wilson and Bandurski (1956), and Robbins and Lipmann (1956, 1958a, b). 

The basic lesion in Gaucher s disease has not yet been elucidated. The storage of cerebrosides, and their identification as glucocerebrosides have stimulated intensive studies on a possible abnormality of cerebroside metabolism. These have... 

Devor, a. W., C. Conger, and I. Gill The use of resorcinol for identification and determination of monosaccharide groups a report on a Gaucher spleen cerebroside. Arch. Biochem. 73, 20 (1958). 

Bjorkman, L.R., Karlsson, K.A., Pascher, I., and Samuelson, B.E. (1972) The identification of large amounts of cerebroside and cholesterol sulfate in the sea star, Asterias rubens. Biochim. Biophys. Acta, 270, 260-265. 

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