Cereal a-amylase

The reason for the non-response of the Hagberg Falling Number to fungal a-amylase is that it is inactivated at 75°C rather than the 87°C of cereal a-amylase. It turns out that fungal a-amylase preparations improve loaf volumes considerably. Most of this effect is produced by a lipoxygenase enzyme that is also present. 

To date, several a-amylases have been identified in diverse organisms, e.g., Aed a 4 in yellow fever mosquito or Der p 4 in European house dust mite. Cereal a-amylases are important allergens for patients with baker s asthma involving the two crucial allergens from barley (Hor v 15 and Hor v 16). Some of these patients also show IgE reactivity to fungal a-amylases, e.g., Asp o 21 from Aspergillus, used as baking additives or present in mold contaminated flour. 

Oryza sativa (Poaceae) [seed] RA5 (14 kDa 10 Cys), RAM (15 kDa 10 Cys) Cereal a-Amylase /Trypsin inhibitor homologue [306]... 

Triticum aestivum (wheat) (Poaceae) Chloroform-methanol soluble proteins CM , CM2, CM3, CM16.CM17 (13-16 kDa 10 Cvs heterotetramers) Cereal a-Amylase /Trypsin inhibitors [308- 311]... 

Triticum aestivum (wheat) (Poaeeae) CMXl, CMX2, CMX3 (11-15 kDa lOCys) Cereal a-Amylase/Trypsin inhibitor homologues [312]... 

Figure 6. Schematic representation of the porcine pancreatic a>amylase a//S-barrel catalytic domain (adapted with permission from Buisson, Duee, Payan, Haser, Food Hydrocolloids 1987,1, 399-406. Copyright 1987, Oxford University Press). Catalytic (O), binding (15) and additional binding residues in Taka-amylase A (14) are indicated ( ), as are 6 conserved regions (1.-6.) (25) and a position for a j9-cyclodextrin binding site (/3-CD) unique to cereal a-amylases (50).

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