Ceratocystis stenoceras

In dimorphic fungi, the composition of constituent polysaccharides is sensitive to morphology and to the cultural conditions. These effects are accentuated in the case of Sporothrix schenckii, and 13C-n.m.r. spectroscopy can be used to detect individual polysaccharides in a qualitative way. The 13C-n.m.r. spectra of mannose-containing polysaccharides of ten Sporothrix schenckii and three Ceratocystis stenoceras species, grown under various conditions, were distinguishable in terms of the presence of signals,130 at 8C 103.3 to 103.7, which arose from 0-a-L-rhamnopyranosyl-(l- 2)-0-a-L-rhamnopyranosyl-(1— 3)-side-chains (14 C-l, 103.7 C-l, 96.8), and were not present in... 

Depicted structures are formed from schenckii in addition to a galactomannan and a dirhamnosylmannan which is similar to A except for an additional O -a-L-rhamnopyranosyl-O—>2)-unit in the side chain (Fig. 11). Structure B is a structure produced from Ceratocystis stenoceras, a yeast that is often found associated with S. schenckii in its natural habitat of vegetation. 

Galactomannan of Ceratocystis stenoceras containing single unit and 3-D-(1 —>6)-1inked D-galactofuranosyl side-chains. 

Figure 9. C-/5 iVAfi spectrum of galactomannan of Ceratocystis stenoceras...

Constit. of the galactomannan isol. from Ceratocystis stenoceras tentatively assigned as a constit. of the galactoman-nans of Sporothrix schenckii and Trichophyton interdigitale. 

Rosa glauca, alginates from Laminaria digitata and L. hyperborea, oligosaccharides released by enzymic hydrolysis of agarose, the rhamnomannans and acidic rhamnomannans of Sporothrix schenckii and Ceratocystis stenoceras, and cellulose 0-methyl, 0-carboxymethyl, and 0-hydroxyethyl ethers. Details of the structures of these polymers are included in Part II of this Report. 

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