Centrifugal chromatography

Physical Methods and Physical Chemistry.—Separation and Assay Methods. A procedure for h.p.l.c. of plant pigments has been used to separate the carotenoids of spinach and of a diatom. H.p.l.c. separations of citrus carotenoids, and of retinal (67) isomers have also been reported. Carotenoid mixtures have also been separated efficiently and rapidly by centrifugal chromatography. ... 

This was separated into two components by centrifugal chromatography (alumina,... 

Monoclonal antibody 0.01-1 Precipitation/centrifugation, chromatography, diafiltration, ultrafiltration [4]... 

In some chemical analyses, it is necessary to separate the analytes present in a mixture, to identify the different analytes and to quantify their amount. Some of the common processes that perform this manipulation of analytes include extraction, precipitation, dialysis, ultrafiltration, distillation, centrifugation, chromatography, and electrophoresis. The last two are the most frequently used separation techniques by those who do chemical analyses of biological fluids. 

Table 3 Phenolic compounds isolated by Centrifugal Chromatography (Chromatotrone).

A Uncomycin standard is treated in the same manner as the samples. Each dry or dried sample is dissolved in 1 ml of pyridine, and 0.2 ml of the silylating reagent is added. The reaction mixtures are aUowed to stand not less than 30 min. 1 ml of the internal standard soln. and 2 ml of water are added, and the mixture is shaken vigorously. The phases are separated by gravity or centrifugation Chromatography and calculations... 

To avoid the above problems we routinely use an extraction method in which DNA fragments become bound to hyroxyapatite powder (9). This is handled in the form of a centrifuged chromatography column for the purposes of washing with various buffers and final elution of pure DNA in 0.5 M potassium phosphate. This DNA is concentrated and desalted in a centrifugal ultrafiltration device (Centricon-10 from Amicon). Many commercial kits are available for the isolation of pure DNA from biological samples therefore a detailed description of the above method seems inappropriate, however, choice of the method should take into account the above considerations. 

Fractionation of TB could be the first example of the use of countercurrent centrifugal chromatography chromatography) with marine natural products (Bruening et al., 1986). 

The isolation of reaction centers requires treatment of the membranes by detergents, followed by classical purihcation methods such as centrifugation, chromatography, etc. (see, e.g., Reference 26). Their polypeptidic composition is determined by dissociation of pure RCs with sodium dodecyl sulfate, followed by gel electrophoresis under denaturing conditions. 

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