Cellulase Onozuka

Enzyme solution Cellulase onozuka RS/Macerozyme R-10 (Yakult Pharmaceuticals) 0.1 g of each enzyme in 1 mL of RNase-free water. 

In 1972 Ogawa and Toyama (56) purified three components— A-I-a, A-I-b, and A-II-1—which were adsorbed on a gauze column during purification from Cellulase Onozuka P1500, a commercial preparation of T. viride cellulase. These three components had molecular weights of 32,000, 48,000, and 48,000 as determined by gel filtration and contained 7-16% carbohydrate. Each is reported to carry out the random hydrolysis of CM-cellulose and to degrade hydrocellulose (Avicel) and cellooligosaccharides except for cellobiose. The order of reactivity toward either cotton or Avicel was A-II-1 > A-I-b > A-I-a. The proteins adsorbed on cellulose comprised 38% of the total cellulase protein. 

Cellulase onozuka PE Meicelase P Cellulosin AP Cellulosin AP with beta-glucanase... 

Enzyme preparation Cellulase onozuka P500 (T. viride) and Macerozyme (Rhizopus... 

For example, almost all carrot pieces were degraded into viscous reddish orange paste, and the residue consisted of cuticle, crude fibers, and other insoluble matter. Production of unicellular sweet potato was exemplified (Table IV) by using Pancellase P500 (equivalent to Cellulase onozuka P500) with or without Macerozyme (33). Sweet potato pieces... 

The authors thanks are due to Kinki Yakult Co. Ltd., Meiji Seika Co. Ltd. and Ueda Chem. Ind. Co. Ltd. for kindly supplying Cellulase onozuka, Macerozyme, Meicelase, and Cellulosin for these studies. The author wishes also to acknowledge support in part by a Grant in Aid for Developmental Scientific Research of the Ministry of Education (1964 and 1965), a Grant in Aid for Fundamental Scientific Research of the Ministry of Education (1966, 1967 and 1968) and with the aid of funds supplied by the Waksman Foundation of Japan Inc. (1960) and by Miyazaki Prefecture (1966). 

Pea epicotyl extract reduces the viscosity of a solution of Capucine xylo-glucans. Results of comparison of the action of this extract with that of a commercial cellulase (Onozuka) were in favour of a hypothesis according to which the cellulase from the extract could hydrolyse the substrate. 

Ion-exchange chromatography of an extract of Cellulase-Onozuka (a commercial ri indepreparation)gavethreecellulasecomponents, one(mol. wt. 5.3 x lO ) of which exhibited both P-o-glucosidase and exo-cellulase activities. " The... 

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