Extracellular cellobiase

These results, taken together with the data reported previously in this chapter, indicate that T. reesei produces one major endoglucanase and one cellobiohydrolase. Cellobiase-hydrolyzing enzymes are probably located intracellularly as well as extracellularly. More work is needed to determine whether or not the intracellular cellobiase is the same as the extracellular cellobiase. 

T. reesei yields one major endoglucanase and one major cellobiohydrolase. The multiplicity of these components is minimal when culture conditions are carefully controlled. The widely reported multiplicity of these components might possibly be due to post-translational modification of one endoglucanase and one cellobiohydrolase. The third major component produced by T. reesei, cellobiase, is present intracellularly as well as extracellularly. Whether or not these two enzyme fractions are the same enzyme, remains to be determined. 

This chapter deals with three aspects of the cellulolytic enzyme system of Thermoactinomyces sp. the location of the CM-cellulase, Avicelase, and / -glucosidase (cellobiase) activities in the culture, the multiplicity of the extracellular enzyme system, and the stability of the different activities as a function of pH, temperature, and time. The results are discussed with reference to saccharification of cellulosic materials. 

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