Enzyme cellobiase-hydrolyzing

These results, taken together with the data reported previously in this chapter, indicate that T. reesei produces one major endoglucanase and one cellobiohydrolase. Cellobiase-hydrolyzing enzymes are probably located intracellularly as well as extracellularly. More work is needed to determine whether or not the intracellular cellobiase is the same as the extracellular cellobiase. 

Enzyme concentration may be expressed in mass units instead of molar units. However, the amount of enzyme is not well quantified in mass units because the actual contents of an enzyme can differ widely depending on its purity. Therefore, enzyme concentration is expressed as an arbitrarily defined unit based on its catalytic ability. For example, one unit of an enzyme, cellobiase, is defined as the amount of enzyme required to hydrolyze cellobiose to produce 1 amol of glucose per minute. Whatever unit is adopted for Ceo, the unit for k CEo should be the same as that of r, kmole/mVs. Care should be taken for the consistency of units when enzyme concentration is not expressed in molar units. 

Hydrolyzing and fermenting microorganisms are responsible for the initial attack on polymers and monomers and produce mainly acetate and hydrogen and some amounts of volatile fatty acids (VFA) such as propionate and butyrate. Hydrolytic microorganisms lead to hydrolytic enzymes, e.g., cellulose, cellobiase, xylanase, amylase, hpase, and protease. 

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