Cell viability shear sensitivity

ITT systems have been used at aU process scales. With the appropriate cleaning validation to demonstrate removal of protein and debris from previous use, tangential flow Alters can be cleaned and used multiple times. ITT is usually not appropriate for clarihcation of a very shear-sensitive cell hne or a low viability process as the turbulent flow required to recirculate the cells in the system leads to more cell breakage, resulting in release of more impurities in the clarified product. 

Such information about shear effects in plant cell suspension cultures as given above is useful for bioreactor design and operation as well as the optimization of bioreactor environments for plant cell cultures. It may also be beneficial to bioreactor scale-up and high-density cultivation of plant cells for production of useful plant-specific chemicals (pharmaceuticals). In addition, because different cell suspensions can show different degrees of cell sensitivity to shear stress, and shear affects culture viability, cell lysis, and even metabolite secretion, as demonstrated in various cases like cell cultures of tobacco, Catharanthus roseus and Perilla frutescens [17, 54, 61], detailed studies are required for individual cases. 

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