Cell surface engineering

Saxon, E., and Bertozzi, C.R. (2000) Cell surface engineering by a modified Staudinger reaction. Science 287, 2007-2010. 

Medof, M. E., Nagargian, S. and Tykocinski, M. L. Cell-surface engineering with GPI-anchored proteins. FASEB J. 10 574-586,1996. 

Conformational restriction of biologically flexible molecules is a successful strategy in drug development. Application of this concept to ceramide, the membrane anchor of sphingolipids, led to the unexpected inhibition of glycosyltransferases involved in the combinatorial biosynthesis of gangliosides. This discovery offers a new approach to cell surface engineering. 

Fig. 1.4.8. Concept for cell surface engineering exogenously added ceramide analogs can lead to modification of the hydrophobic and the hydrophilic part of membrane sphingolipids and alter the properties of membrane proteins.

Metabolic cell-surface engineering to promote the endocytic uptake of ligands... 

Hang HC, Bertozzi CR. Ketone isoteres of 2-V-acetamido-sugars as substrates for metabolic cell surface engineering. J. Am. Chem. Soc. 2001 123 1242-1243. 

M. Osumi, T. Amachi, A. Tanaka, Construction of a starch-utilizing yeast by cell surface engineering, Appl. Environ. Microbiol. 1997, 63(4), 1362-1366. 

Medof M.E., Nagarajan, S., Tykocinski, M.L. (1996) Cell-surface engineering with GPl-an-chored proteins. FASEB J. 10, 574-586. 

Yeast Cell-Surface Engineering System for Biomass Utiiization... 

Yeast cell-surface engineering has been established to display enzymes, functional proteins, antibodies, and combinatorial protein libraries (Kondo and Ueda, 2004). The cell surface is a functional interface between the inside and outside of the cell allowing some surface proteins to extend across the plasma membrane, while others are bound by non-covalent or covalent interactions to the cell surface components. For anchoring surface-specific proteins, yeast cells have molecular systems to confine... 

Application of Cell-Surface Engineering to Thermotolerant Yeast... 

Hasunuma T, Kondo A. (2012b). Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering. Biotechnol Adv, 30, 1207-1218. 

Matano Y, Hasunuma T, Kondo A. (2013b). Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain. Appl Microbiol Biotechnol, 97, 2231-2237. 

Ueda M, Tanaka A. (2000). Cell surface engineering of yeast constraction of arming yeast with biocatalyst. J Biosci Bioeng, 90,125-136. 

Chapter 5.3 Cell Surface Engineering via Methacryloyl-Derivatized Carbohydrates 255... 

Next, in order to demonstrate the efficacy of this cell surface engineering approach, cell microarrays were prepared using a micropattemed poly(2-methacryloyloxyethyl phosphorylcholine [MPC]) (PMPC) polymer brush surface as the substrate. PMPC brushes were prepared on a silicon (Si) wafer via surface-initiated atom transfer radical polymerization, as previously described elsewhere [35]. Selective decomposition of the initiators immobilized on the Si wafer using UV light irradiation resulted in micropatterning of the... 

---