Cell sheet-based regenerative medicine

Oral mucosal cell sheets are also clinically applied to the treatment of esophageal ulcerations using an endoscopic transplantation method (Kanai, Yamato, Ohki, Yamamoto, Okano, 2012 Ohki et al., 2006, 2012). Removal of large esophageal cancer tissue section by endoscopic submucosal dissection causes postoperative inflammation and stenosis from a lack of an epithelial layer. After the transplantation 

Lung air leak followed by pulmonary resection was attempted to be sealed by skin fibroblast sheets in an animal model (Kanzaki et al., 2007,2008,2012). In clinical use. 

Switchable and Responsive Surfaces and Materials for Biomedical Applications 

Cell-sheet based tissue engineering has been further applied to the treatment of complexes and functional tissues and organs such as a bladder (Shiroyanagi, Yamato, Yamazaki, Toma, Okano, 2003, 2004), liver (Ohashi et al., 2007) and islets (Shimizu et al., 2009). 

Mice hepatic-layered cell sheets, which express human a 1-antitrypsin, are transplanted into mice subcutaneous locations with neovascular networks (Ohashi et al., 2007). After transplantation, liver-specific functions such as albumin production and drug metabolism are improved and kept for longer than 200 days. In addition, the transplanted cell sheet has an ability to proliferate and grow in response to a regenerative stimulus, which is induced by the resection of two-thirds of the host liver (Ohashi et al., 2007). 

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