Cell permeation chromatography

ApoA-IV is an immunologically distinct apolipoprotein of Mr 46,000 (B22, G28, W7). It has been demonstrated in intestinal epithelial cells from fasting subjects and a marked increase has been shown during lipid absorption (G27). About 10-13% of chylomicron apolipoprotein and 24-30% of intestinal VLDL apolipoprotein is apoA-IV. In fasting plasma, 98% of apoA-IV is in the d> 1.21 g/ml fraction and in lipemic plasma 90% is in this fraction, while 10% is associated with triglyceride-rich lipoproteins (G27). Gel permeation chromatography confirmed that in plasma most apoA-IV is free, unassociated with lipoproteins (B22, G27). 

Vapors of acrylonitrile were adsorbed into the dehydrated forms of different large- and medium-pore zeolites, to saturation levels of 46, 6, and 9 molecules of acrylonitrile per unit cell in dehydrated zeolite NaY, Na-mordenite, and silicalite, respectively. Subsequent reaction with radical initiator (aqueous solution of K2S2O8 and NaHSOs) produced intrazeolite polyacrylonitrile (no polymer was found in silicalite due to size constraints). The intrazeolite polyacrylonitrile could be recovered after dissolution of the host with dilute aqueous HF, and was very similar to bulk polyacrylonitrile. Gel permeation chromatography revealed a peak molecular weight of 19,000 for polyacrylonitrile recovered from the NaY host, and about 1,000 for the polymer from mordenite. 

Fig. 5. Gel-permeation chromatography (on Bio-Gel P-2) of soluble extracellular material from spinach cell suspension cultures that had been fed L-[l- H]arabinose [32]. (a). Total H-labelled material in the culture filtrate - mainly [ Hjpolysaccharides (K. =0.00) and unincorporated [ HJarabinose (K,=0.96). (b) The intermediate fractions (shown in black in Fig. a) were pooled, concentrated and le-chromatographed on the same column and the fractions were assayed specifically for [ HjXGOs.

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