Cell lysates homogenate

Blank reaction without cell lysate mix 25 pi of homogenization buffer, 74 pi of reaction buffer and 1 pi of 100 mM GTP and proceed by starting the incubation for 1 h at 37°C in the dark, as described for the reaction mixture. 

Homogenates and cell lysates from Escherichia coli BL21 (DE3) strain harboring in the empty pET 15b plasmid were used as a control for these enzymatic activity assays. 

Gene products in red-cell lysates and kidney homogenates are analyzed with gel electrophoresis. Almost all Fis survive the surgical procedures used to get samples and are thus available for further breeding analysis. Untreated control groups are examined in the same way. 

Transfer cell suspension to glass potter tube and homogenize using a rotor-connected Teflon pesde (1100-1200 rpm) by 10 strokes (up and down). Cell lysate is transferred to conical polypropylene tubes. 

Kinase Profiling in Cell Lysates and Tissue Homogenates... 

Disrapt tissue or cells and homogenize lysate in 600pL RLT buffer. 

If the matrix is a biological tissue for which it is desired to disrupt the cells and homogenize the resulting lysate, ultrasonic disruption is widely used, exploiting the cavitation phenomenon discussed in Section 4.3.2c. That earlier discussion was mainly concerned with extracting analytes from slurries of small particles and by implication referred mainly to thermally stable analytes. When... 

Cell lysates of "resting" human peripheral blood lymphocytes incorporate 2-3 nmol glycine mg protein h into purine ribonucleotides, one-twentieth the activity observed in avian liver homogenates. Lectin (phytohemagglutinin) activation of cultured lymphocytes results in a fifteen to twenty-fold increase in total activity and a four to five-fold increase in specific activity after 72 h, with equivalent changes in the activity of the first pathway enzyme amidophosphoribosyltransferase (EC 2.4.2.14). 

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