Cell function modifications

E9. Endo, S., Inada, K., Yamada, Y., Takakuwa, T., Nakae, H., Kasai, T Koike, S., Inoue, Y., Nii-mi, M Wakabayashi, G and Taniguchi, S Functional modification of vascular endothelial cells by cytokines during septic shock. Res. Commun. Mol. Pathol. Pharmacol. 96, 23-38 (1996). 

There is also a need for cell systems that express a multiplicity of transporters. Cell line modification through cDNA-expression can be used to add missing functions to cells that are known to express only a subset of the enzymes or transporters that are needed and are known to be present in vivo. In this way, in vitro screening models can be improved. 

Nigo YI, Yamashita M, Hirahara K, et al Regulation of allergic airway inflammation through Toll-like receptor 4-mediated modification of mast cell function. Proc Natl Acad Sci USA 2006 103 2286-2291. 

Powers, S., et al. (1986). RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell 47 413 22. 

O Donnell N, Zachara NE, Hart GW, Marth ID. Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol. Cell. Biol. 2004 24 1680-1690. 

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