Cell cycle analysis by flow cytometry

Furthermore, cell cycle analysis by flow cytometry can be improved by using other complementary techniques that provide additional information. This is the case of the mitotic index that informs us about the number of cells undergoing mitosis during the experiment. This measurement was also recorded in our investigation, and the results indicated its excellent complementary information. 

After incubation started, cell cycle analysis by flow cytometry and cell cycle histograms were recorded for BOA-treated and control plants every 2 h, until 12 or 14 h of incubation with BOA. At least 10,000 nuclei from each sample must be analyzed in the flow cytometer. 

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