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Cell culture carrier

For thermo-responsive cell culture carriers the transition temperature of the SRP coating should be slightly below 37°C to allow cell adhesion under standard culture conditions and cell detachment with a rather small temperature decrease. Pure... [Pg.252]

Most of the preparation techniques mentioned above allow lateral patterning of thermo-responsive cell culture carriers by simple masking techniques. This includes ultraviolet (Chen et al. 1998) and electron beam irradiation (Tsuda et al. 2005) as... [Pg.255]

For applications aiming at the control of marine biofouUng, a significantly reduced phase transition temperature compared to the PNiPAAm homopolymer is required. For that purpose, Cordeiro et al. (2009) sythesised copolymers of NiPAAm with N-(1-phenyl ethyl)acrylamide 10-15 mol% of this hydrophobic unit leads to a shift of more than 10 K. Another example for lowering the phase transition tanperature is found in the work of Tsuda et al. (2004). NiPAAm-based copolymers with different amounts of the hydrophobic unit n-butyl methacrylate were studied to fabricate tunable thermo-responsive cell culture carriers. As shown in Figure 6.1, a pronounced shift of the phase... [Pg.149]

Beyond physicochemical approaches, switchable cell culture carriers can be provided with a biomolecular functionalisation to more effectively tune attachment, growth and proliferation as well as detachment of adherent cells. To allow covalent protein or peptide immobilisation, reactive units are introduced into the thermo-responsive material. [Pg.152]

Another route towards biomolecular functionalisation of switchable cell culture carriers was proposed by Gramm et al. (2011). The thermo-responsive polymer poly (vinyl methyl ether) was blended with a small amount of the alternating copolymer of vinyl methyl ether and maleic anhydride. After electron beam cross-linking, stable films with a dry thickness of up to 70 nm were obtained. The introduced anhydride moieties allow for subsequent protein or peptide immobilization without toxic coupling agents. This concept was applied and further developed by Teich-mann et al. (2013). The impact of different adhesion-promoting molecules immobilised in different concentrations to the thermo-responsive coating was evaluated and compared. [Pg.152]

Teichmann, J., Valtink, M., Gramm, S., Nitschke, M., Werner, C., Funk, R., et al. (2013). Human comeal endothelial cell sheets for transplantation thermo-responsive cell culture carriers to meet cell-specific requirements. Acta Biomaterialia, 9,5030-5038. [Pg.164]

The introduced method for the preparation and in situ attachment of fibrillar collagen and glycosaminoglycans assemblies to reactive polymer coatings comprises an efficient technique for a straightforward biofunctionahza-tion of cell culture carriers and medical devices. As a basic advantage the... [Pg.87]


See other pages where Cell culture carrier is mentioned: [Pg.76]    [Pg.249]    [Pg.250]    [Pg.251]    [Pg.252]    [Pg.252]    [Pg.254]    [Pg.255]    [Pg.262]    [Pg.281]    [Pg.84]    [Pg.191]    [Pg.192]    [Pg.87]    [Pg.150]    [Pg.65]    [Pg.86]    [Pg.150]   
See also in sourсe #XX -- [ Pg.84 ]




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