CDs in affinity chromatography

Similar separation behavior emerges in the case of debranching enzyme such as puUulanase, and the interaction occurring here is also at the active sites of such enzymes. For example, the starch-debranching enzymes from spinach leaf is loaded in /3-CD Sepharose 6B and washed with sodium acetate buffer to remove other enzymes, then the retarded starch-debranching enzyme is released with the elution solution including fS-CD. Actually, /3-CD exhibit affinity for all types of 

As for Qf-amylase, there are some reports that some a-amylases of wheat and triticale can be purified by f)-CD Sepharose 6B. But it is of interest that the interaction with the CD occurs at the raw starch affinity site rather than the active site of the enzyme, which means j8-CD did not inhibit soluble starch hydrolysis but only the raw starch hydrolysis of the enzymes [49,50]. 

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