CDAA metabolism

Effect of CDAA pretreatments on subseauent CDAA metabolism... 

Although CDAA pretreatments elevated both GSH and GSH-S.-transferase activity (as assayed by CDNB), it was essential to determine whether these effects would actually result in greater CDAA metabolism. For these studies, we examined [14C]CDAA metabolism in vitro and in excised corn root tips [detailed methods described by Ezra et. al. (20)]. The in vitro assay revealed a significant level of non-enzymatic [A C] CDAA... 

However, in all experiments, pretreated roots took up 2-fold more [14C]CDAA and metabolized it twice as fast as non-pretreated root tissue. 

In a series of experiments, similar to those with CDAA, R-25788 and EPTC were examined as pretreatments to either enhance their own metabolism by GSH-S.-transf erase or prevent later injury (from toxic doses) in corn seedlings. 

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