Cation exchange chromatograph

Simultaneous determination of both cations and anions in acid rain has been achieved using a portable conductimetric ion-exclusion cation-exchange chromatographic analyzer.14 This system utilized the poly(meth-ylmethacrylate)-based weak acid cation exchange resin TSK-Gel OA-PAK-A, (Tosoh , Tokyo, Japan) with an eluent of tartaric acid-methanol-water. All of the desired species, 3 anions and 5 cations, were separated in less than 30 minutes detection limits were on the order of 10 ppb. Simultaneous determination of nitrate, phosphate, and ammonium ions in wastewater has been reported utilizing isocratic IEC followed by sequential flow injection analysis.9 The ammonium cations were detected by colorimetry, while the anions were measured by conductivity. These determinations could be done with a single injection and the run time was under 9 minutes. 

Tanaka, K., Ohta, K., Haddad, P. R., Fritz, J. S., Lee, K.-R, Hasebe, K., Ieuji, A., and Miyanaga, A., Acid-rain monitoring in East Asia with a portable-type ion-exclusion cation-exchange chromatographic analyzer, ]. Chromatogr. A, 850, 311, 1999. 

Stein reported the separation of choline and acetylcholine by a cation-exchange chromatographic method [198]. Four columns were compared by Salamoun et al. for the separation of choline and acetylcholine by cation exchange liquid chromatography [178]. The mobile phase used was... 

Ibayashi, J., Clinical studies on gastric juice protein. On cation exchange chromatographical analysis of gastric juice protein. Sapporo Med. J. 15, 231-246 (1959). 

Various gel- and porous-type resins have been examined for use in the cation-exchange chromatographic separation of the transplutonium elements from the fission-product lanthanides with an eluent of 11.7 M hydrochloric acid (17). In the case of gel-type resins, very fine ones such as colloidal aggregate, are needed to perform good separation. The number of theoretical plate obtained... 

Saifer, A., A Zary, E. A., Valenti, C. and Schneck, L., Quantitative cation-exchange chromatographic analysis of free amino acids in human amniotic fluid collected during early pregnancy. Clin. Chem. 16, 891-895 (1970). 

The characteristics of the iodide salts are either identical (cation exchange chromatographic behavior) or close (IR and electronic spectra, solubilities) to those of the related bromides (Section A). 

TABLE 3 Cation Exchange Chromatographic Separation of Surfactants... 

The results of this study demonstrate that use of a positive cation-exchange chromatographic step can be used to significantly enrich the content of a monoclonal antibody from a tissue culture medium in a single step. The monoclonal antibody could... 

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