Caspases during apoptosis

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A large number of caspase substrates have been identified, some of which have a direct relationship to survival of the cell. The following examples illustrate important cellular processes and substrates that are targeted for proteolysis during apoptosis Inactivation of proteins that protect from apoptosis ... 

Earnshaw, W.C., Martins, L.M. and Kaufmann, S.H. Mammalian Caspases Structure, Activation, Substrates and Functions during Apoptosis (1999) Ann. Rev. Biochem. 68, 383-424. 

The TNF-a-induced DNA fragmentation may have been caused by caspase-activated deoxyribonuclease (CAD) in PC-12 cells. In non-apoptotic cells, CAD is present as an inactive complex with the inhibitor jOvd p3,24]. During apoptosis, caspase-3 inactivates ICAD, leaving CAD free to function as a nuclease [25]. Therefore, we used die fluorogenic substrate, Ac-DEVD-MCA, to determine whether the caspase-3 in PC-12 cells was activated by treatment with TNF-a and/or crocin. As shown in... 

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